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double antibody sandwich elisa kit  (Elabscience Biotechnology)


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    Structured Review

    Elabscience Biotechnology double antibody sandwich elisa kit
    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using <t>ELISA.</t> (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
    Double Antibody Sandwich Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sandwich+elisa/Human+AMBP+(Alpha-1-Microglobulin%2FBikunin+Precursor)+ELISA+Kit/pmc13124742-121-7-11
    Average 94 stars, based on 1 article reviews
    double antibody sandwich elisa kit - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning"

    Article Title: Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning

    Journal: Biochemistry and Biophysics Reports

    doi: 10.1016/j.bbrep.2026.102593

    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
    Figure Legend Snippet: AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

    Techniques Used: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing

    Related Articles

    Sandwich ELISA:

    Article Title: Exploring the Association of VEGF-A and ANGPTL2 with the Prognosis of Non-proliferative and Proliferative Diabetic Retinopathy
    Article Snippet: The serum ANGPTL2 was measured using sandwich ELISA (elabscience cat no. E-EL-H6034) following the manufacturer’s instructions.

    Article Title: INF-&lt;i&gt;&amp;#947;&lt;/i&gt; and IL-2 Profile as Therapeutic Markers in Tuberculosis Patients at the Jamot Hospital of Yaounde-Cameroon
    Article Snippet: Biological analysis of samples: Cytokines quantification was performed using a sandwich ELISA (Enzyme Linked-Immunosorbent Essay), with Elabsciences kits for human INF-γ and human IL-2, according to manufacturer instructions.

    Article Title: Tumor treating fields alter local coagulation dynamics in glioblastoma patients.
    Article Snippet: TF abundance on EVs was quantified using a sandwich ELISA (Elabscience®, Catalog No: E-EL-H0040) with EVs, following the manufacturer's protocol.

    Article Title: Tumor treating fields alter local coagulation dynamics in glioblastoma patients
    Article Snippet: TF abundance on EVs was quantified using a sandwich ELISA (Elabscience®, Catalog No: E-EL-H0040) with EVs, following the manufacturer's protocol.

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    Article Title: Unmasking metabolic clues: adipsin, irisin and osteopontin as biomarkers in polycystic ovary syndrome and their impact on metabolic dynamics: a case-control study.
    Article Snippet: Irisin levels were measured using a sandwich ELISA (Elabscience, catalog no. E-EL-H6120) with intra-assay CVs of 5.8% and 6.7%, along with a sensitivity of 0.09 ng/mL [7].

    Article Title: Unmasking metabolic clues: adipsin, irisin and osteopontin as biomarkers in polycystic ovary syndrome and their impact on metabolic dynamics: a case-control study.
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    Article Title: Maternal Serum SCUBE-1: A Novel Ischemic Marker in Preeclampsia
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    Inter Assay:

    Article Title: Exploring the Association of VEGF-A and ANGPTL2 with the Prognosis of Non-proliferative and Proliferative Diabetic Retinopathy
    Article Snippet: The serum ANGPTL2 was measured using sandwich ELISA (elabscience cat no. E-EL-H6034) following the manufacturer’s instructions.

    Article Title: INF-&lt;i&gt;&amp;#947;&lt;/i&gt; and IL-2 Profile as Therapeutic Markers in Tuberculosis Patients at the Jamot Hospital of Yaounde-Cameroon
    Article Snippet: Biological analysis of samples: Cytokines quantification was performed using a sandwich ELISA (Enzyme Linked-Immunosorbent Essay), with Elabsciences kits for human INF-γ and human IL-2, according to manufacturer instructions.

    Article Title: Tumor treating fields alter local coagulation dynamics in glioblastoma patients.
    Article Snippet: TF abundance on EVs was quantified using a sandwich ELISA (Elabscience®, Catalog No: E-EL-H0040) with EVs, following the manufacturer's protocol.

    Article Title: Tumor treating fields alter local coagulation dynamics in glioblastoma patients
    Article Snippet: TF abundance on EVs was quantified using a sandwich ELISA (Elabscience®, Catalog No: E-EL-H0040) with EVs, following the manufacturer's protocol.

    Article Title: Nanoparticles from grape seed extract inhibit inflammatory cytokines and ameliorate CCl 4 -induced hepatotoxicity
    Article Snippet: Serum CRP was measured using commercial sandwich ELISA (Catalog number: E-EL-R0506, Elabscience ® , Texas, USA).

    Article Title: Unmasking metabolic clues: adipsin, irisin and osteopontin as biomarkers in polycystic ovary syndrome and their impact on metabolic dynamics: a case-control study.
    Article Snippet: Irisin levels were measured using a sandwich ELISA (Elabscience, catalog no. E-EL-H6120) with intra-assay CVs of 5.8% and 6.7%, along with a sensitivity of 0.09 ng/mL [7].

    Article Title: Unmasking metabolic clues: adipsin, irisin and osteopontin as biomarkers in polycystic ovary syndrome and their impact on metabolic dynamics: a case-control study.
    Article Snippet: Serum osteopontin levels were measured using a sandwich ELISA (Elabscience, catalog no. E-ELH1347) with intra- and inter-assay CVs of < 4.1% and < 6.7% [16].

    Article Title: Maternal Serum SCUBE-1: A Novel Ischemic Marker in Preeclampsia
    Article Snippet: Serum SCUBE-1 concentrations were determined by using commercially available kits based on a sandwich-ELISA (Elabscience, Cat No: E-EL-H5405, Lot: Y5GV5ZEU8U, Hubei, Wuhan, China) method, and the results were expressed as ng/mL.

    Intra Assay:

    Article Title: Exploring the Association of VEGF-A and ANGPTL2 with the Prognosis of Non-proliferative and Proliferative Diabetic Retinopathy
    Article Snippet: The serum ANGPTL2 was measured using sandwich ELISA (elabscience cat no. E-EL-H6034) following the manufacturer’s instructions.

    Article Title: INF-&lt;i&gt;&amp;#947;&lt;/i&gt; and IL-2 Profile as Therapeutic Markers in Tuberculosis Patients at the Jamot Hospital of Yaounde-Cameroon
    Article Snippet: Biological analysis of samples: Cytokines quantification was performed using a sandwich ELISA (Enzyme Linked-Immunosorbent Essay), with Elabsciences kits for human INF-γ and human IL-2, according to manufacturer instructions.

    Article Title: Tumor treating fields alter local coagulation dynamics in glioblastoma patients.
    Article Snippet: TF abundance on EVs was quantified using a sandwich ELISA (Elabscience®, Catalog No: E-EL-H0040) with EVs, following the manufacturer's protocol.

    Article Title: Tumor treating fields alter local coagulation dynamics in glioblastoma patients
    Article Snippet: TF abundance on EVs was quantified using a sandwich ELISA (Elabscience®, Catalog No: E-EL-H0040) with EVs, following the manufacturer's protocol.

    Article Title: Nanoparticles from grape seed extract inhibit inflammatory cytokines and ameliorate CCl 4 -induced hepatotoxicity
    Article Snippet: Serum CRP was measured using commercial sandwich ELISA (Catalog number: E-EL-R0506, Elabscience ® , Texas, USA).

    Article Title: Unmasking metabolic clues: adipsin, irisin and osteopontin as biomarkers in polycystic ovary syndrome and their impact on metabolic dynamics: a case-control study.
    Article Snippet: Irisin levels were measured using a sandwich ELISA (Elabscience, catalog no. E-EL-H6120) with intra-assay CVs of 5.8% and 6.7%, along with a sensitivity of 0.09 ng/mL [7].

    Article Title: Unmasking metabolic clues: adipsin, irisin and osteopontin as biomarkers in polycystic ovary syndrome and their impact on metabolic dynamics: a case-control study.
    Article Snippet: Serum osteopontin levels were measured using a sandwich ELISA (Elabscience, catalog no. E-ELH1347) with intra- and inter-assay CVs of < 4.1% and < 6.7% [16].

    Article Title: Maternal Serum SCUBE-1: A Novel Ischemic Marker in Preeclampsia
    Article Snippet: Serum SCUBE-1 concentrations were determined by using commercially available kits based on a sandwich-ELISA (Elabscience, Cat No: E-EL-H5405, Lot: Y5GV5ZEU8U, Hubei, Wuhan, China) method, and the results were expressed as ng/mL.



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    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using <t>ELISA.</t> (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
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    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using <t>ELISA.</t> (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.
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    Image Search Results


    AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

    Journal: Biochemistry and Biophysics Reports

    Article Title: Screening of metabolic-related biomarkers linking intervertebral disc degeneration and type 2 diabetes based on comprehensive bioinformatics analysis and machine learning

    doi: 10.1016/j.bbrep.2026.102593

    Figure Lengend Snippet: AMBP promotes imbalance of BCAA metabolism. (A) mRNA transcripts of AMBP in patients with different grades of IVDD and relative quantitative analysis. (B) mRNA transcripts of AMBP in TNF-α-stimulated HNPC cells and relative quantitative analysis. (C) Detection of inhibition efficiency of small interfering RNAs against different targets. (D) Serum concentrations of AMBP in normal, T2DM, IVDD, and T2DM combined with IVDD patients were examined using ELISA. (E) Representative β-gal staining images of HNPC cells before and after AMBP inhibition. (F) Correlation analysis between AMBP expression and β-gal expression. (G) mRNA transcripts of BCAA metabolism-related enzymes in TNF-α-stimulated HNPC cells before and after AMBP inhibition and relative quantitative analysis.

    Article Snippet: Cytokine quantification was performed using a commercial double-antibody sandwich ELISA kit (Elabscience, E-EL-H0315, AMBP) according to the manufacturer's instructions.

    Techniques: Inhibition, Enzyme-linked Immunosorbent Assay, Staining, Expressing